The direction, IN/OUT, is the direction of the final move of the focuser. More info in our docs:
http://mainsequencesoftware.com/Content/SGPHelp/Focusers.html
In most cases you want to use "IN" as this will pull the draw tube "IN" which is against gravity (in the non reversed case). For a reversed focuser you'd want to use OUT to have the last move done against gravity. However reversed focusers and backlash don't play nice with autofocus as you'd be invoking backlash compensation quite a bit. If possible I would recommend reversing the direction of the focuser in the ASCOM driver if they have that capability.
Thanks,
Jared